产品标准

 浓缩液(1mg/1ml) 亲和纯化抗体

 

产品应用

WB=1:100-500 Elisa =1:200-1000 IP=1:20-100 IHC=1:100-500    （石蜡切片需做抗原修复）

 

产品介绍

       肿瘤坏死因子是巨嗜细胞感染时或应激情况下自然产生的一种十分活跃的生物活性因子，可能与干扰素协同作用杀伤瘤细胞。
       Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
        Also known as:
       tumor necrosis factor-α；APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a ; TNF alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precurso; Tumour Necrosis Factor Alpha