产品标准
浓缩液(1mg/1ml) 亲和纯化抗体
产品应用
WB=1:100-500 Elisa =1:200-1000 IP=1:20-100 IHC=1:100-500 （石蜡切片需做抗原修复）
产品介绍
激酶和磷酸酶（Kinases and Phosphatases）
P-tyr蛋白酪氨酸磷酸酶1B（PTP1B）是一种胰岛素信号的负性调节因子，与肥胖症和2型糖尿病的发病及发展关系密切。对P-tyr基因敲除小鼠的研究表明，缺失PTP1B小鼠的胰岛素敏感性明显增加，并对肥胖有一定的抵抗作用，而另一些对动物和人体的研究结果却与此相反，发现PTP1B可通过引起胰岛素抵抗、瘦素抵抗及影响脂代谢等而导致肥胖症和2型糖尿病的发生。一些特异性的Ptyr小分子抑制剂已经开发并用于肥胖和糖尿病的治疗中。
PTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and is a drug discovery target in the fields of diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B.
PTP1B also interacts with Insulin and EGF receptors, and undergoes phosphorylation after receptor stimulation. Tyrosine phosphorylation at Tyr-66, Tyr-152, and Tyr-153 occurs after insulin receptor activation, and tyrosine phosphorylation of Tyr-152 may be required for interactions with N-Cadherin. In addition, Akt can phosphorylate Ser-50 and this phosphorylation can reduce PTP1B activity.
mol wt:49kDa
Other Aliases:
Non receptor tyrosine phosphatase 1; Protein tyrosine phosphatase 1B; Protein tyrosine phosphatase non receptor type 1; Protein tyrosine phosphatase placental; PTP 1B; PTPN 1; PTPN1; Tyrosine protein phosphatase non receptor type 1
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