产品标准:2ml体积/5ml体积
产品介绍:
美国GE公司生产的Ni Sepharose™ 6 Fast Flow具有特异性好、稳定性高、镍离子脱落少、层析介质颗粒粒度均匀、流速快、His标记蛋白结合能量高、使用寿命长、重复性好、质量稳定等特点。 该产品有2ml和5ml两种规格,每ml柱床体积可吸附40mgHis标记蛋白质,可配合注射器或连接液相色谱系统使用。
TECHNICAL SPECIFICATIONS |
Matrix |
Highly crossed-linked 6% agarose, precharged with Ni2+ |
Average particle size |
90 µm |
Dynamic binding capacity* |
Approx. 40 mg histidine-tagged protein/ml medium |
Metal ion capacity |
Approx. 15 µmol Ni2+/ml medium |
Compatibility during use |
Stable in all commonly used buffers, reducing agents, denaturants, and detergents, see Introduction to Tagged Protein Purification for more information. |
Chemical stability |
For one week at 40°C: 0.01 M HCl, 0.1 M NaOH For 12 h: 1 M NaOH, 70% acetic acid 30 min tested: 30% 2-propanol 1 h tested: 2% SDS |
pH stability† |
2-14 (short term), 3-12 (long term) |
Storage |
20% ethanol |
Storage temperature |
4°C to 30°C |
* Protein binding capacity is protein-to protein dependent † Ni2+-stripped medium |
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